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applicator that prevents the gel from entering the vacuum line) freeze2trim After the vacuum suction was started, the applicator was placed on the marked treatment area and then placed on the skin freeze2trim .The vacuum attracted the target tissue to the cup, filling it to a total depth of 2 cm freeze2trim .The vacuum force adhered the applicator to the treatment area with minimal discomfort, and fabric adhesive tapes were attached to a pillow to provide additional support throughout the cooling treatment freeze2trim At the end of the treatment cycle, the applicator was removed and the treatment area was manually massaged for 2 minutes, allowing the tissue to warm up and recover its original shape freeze2trim .At the first treatment visit, the 14 participants received 2 bilateral treatment cycles, overlapping approximately 20% freeze2trim On the second treatment visit, 12 participants received 2 bilateral cycles and 2 participants received 1 focused cycle because it was considered that they did not have enough submental fat remaining to allow the placement of 2 treatment cycles freeze2trim.
Participant Satisfaction Ratings
Participant satisfaction data were collected through the use of a written questionnaire at the 12-week follow-up visit after treatment freeze2trim .This questionnaire used a 5-point Likert scale to obtain answers, but also allowed free text responses freeze2trim.
Caliper Measurement
Before treatment and at 6 and 12 week follow-up visits, clamp measurements were made with the participant’s head held in a neutral position freeze2trim.
The caliper (Defender Body Fat Caliper, Sequoia Fitness Products) was placed to pinch the skin to 15 mm on each side of the center point of the submental area freeze2trim .Three measurements were made and averaged at each of the initial and final follow-up visits freeze2trim. The mean reduction of the fat layer was calculated and statistical significance was determined by a paired 2-tailed t-test freeze2trim P < freeze2trim05 was considered significant freeze2trim.
freeze2trim 